Methods of producing 7-carbon chemicals via aromatic compounds
AssigneeINVISTA North America S.a r.I.
InventorsPaul S. Pearlman, Alex Van Eck Conradie, Changlin Chen, Adriana Leonora Botes
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Abstract
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-CoA degradation pathway enzymes.
Claims23
-42. (canceled)
A method for biosynthesizing a product selected from pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, and 1,7-heptanediol, said method comprising
- (a) enzymatically synthesizing a seven carbon chain aliphatic backbone from benzoyl-CoA, and
- (b) enzymatically forming two terminal functional groups selected from carboxyl, amine, and hydroxyl groups in said backbone, thereby forming the product, wherein
- a thioesterase classified under EC 3.1.2.-, an aldehyde dehydrogenase classified under EC 1.2.1.-, a 7-oxoheptanoate dehydrogenase classified under EC 1.2.1.-, a 6-oxohexanoate dehydrogenase classified under EC 1.2.1.63, a glutaconate CoA-transferase classified under EC 2.8.3.12, or a succinate CoA-ligase classified under EC 6.2.1.5 enzymatically forms a terminal carboxyl group,
- a ω-transaminase classified under EC 2.6.1.-, or a deacetylase classified under EC 3.5.1.62 enzymatically forms an amine group, and/or
- a 6-hydroxyhexanoate dehydrogenase classified under EC 1.1.1.258, a 5-hydroxypentanoate dehydrogenase classified under EC 1.1.1.-, a 4-hydroxybutyrate dehydratase classified under EC 1.1.1.-, or an alcohol dehydrogenase classified under EC 1.1. 1.- enzymatically forms a hydroxyl group.
Dependencies are inferred from the claim text.