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US 2017/0233771 A1Published application
Yeast preparations and methods of making the same
AssigneeButamax Advanced Biofuels LLC
InventorsLarry Cameron Anthony, Caroline Peres, Robert Balcarcel
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Abstract
The invention relates to the fields of industrial microbiology and alcohol production including production of yeast products with features suitable for transport, storage, and utilization in fermentation
Claims21
A method of generating a butanologen yeast product comprising:
- a) culturing a population of butanologen yeast in the presence of a carbon substrate whereby the feed rate of carbon substrate is ramped such that concentration of carbon substrate maintains the growth rate of the population at less than μcrit and whereby the population reaches a cell density;
- b) ceasing the ramping of the feed rate whereby the concentration of carbon substrate decreases over a period of time;
- and
- c) subjecting the population of butanologen yeast to a feed rate which is ramped down to less than half of the maximum feed rate of (a).
1 more element
A recombinant yeast cell comprising:
- a) an engineered butanol biosynthetic pathway;
- and
- b) an engineered trehalose pathway.
Dependencies are inferred from the claim text.