Methods and compositions for t cell generation and uses thereof
AssigneesWashington University, California Institute of Technology
InventorsHerbert W. Virgin, IV, Hiutung Chu, Sarkis K. Mazmanian
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Abstract
Among the various aspects of the present disclosure is the provision of a method of treating an inflammatory bowel disease. Another aspect provides the provision of a screening method for therapeutic agents. Another aspect provides a method of detecting Treg cells to determine if a subject has an inflammatory disease. The present disclosure provides for methods of treatment, diagnosis, and screening for drugs for colitis and ileitis.
Claims20
A method of treating an inflammatory disease or condition comprising:
- administering a therapeutically effective amount of a Treg generating agent to a subject in need thereof, wherein
- the subject exhibits a diminished capacity to generate Treg cells prior to administration;
- and
- Treg generation of the subject is enhanced after administration.
A method of detecting a number of Treg cells generated in a subject comprising:
- (i) obtaining a sample from a subject suspected to be afflicted with an inflammatory disease, disorder, or condition;
- contacting the sample with the Treg generating agent;
- or
- (a) isolating Treg precursors from the sample and (b) contacting the sample with B. fragilis OMVs or a Treg generating agent;
- and
- detecting the number of Treg cells generated;
- or.
A method of screening for a compound capable of restoring Treg generation to a subject, comprising:
- (I) (a) incubating a wild type dendritic cell, an Atg gene-mutant dendritic cell, a wild type macrophage, or an Atg gene-mutant macrophage with Treg precursors in the presence of at least one test compound or salt thereof;
- and
- (b) detecting a level of Treg cells generated;
- or
- (c) detecting a level of cytokine secretions;
- or
- (II) (a) obtaining proteins associated with Treg generation;
- (b) incubating the proteins associated with Treg generation in the presence of at least one test compound or salt thereof;
- and
- (c) detecting interactions of chemicals or chemical moieties.
Dependencies are inferred from the claim text.