Production of Acrylate in Cells
OwnerJameson K. Rogers, George M. Churchinventors
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Abstract
A method of producing acrylate in vivo in a genetically modified bacteria cell is provided including providing a cell with a first one or more nucleic acids encoding a pathway resulting in the production of 3-hydroxypropionate, providing the cell with a second one or more nucleic acids encoding a truncated pcs enzyme lacking a domain converting acrylyl-CoA to propionyl-CoA, providing the cell with a third one or more nucleic acids encoding acrylyl-CoA hydrolase, wherein the cell expresses the first, second and third one or more nucleic acids and produces acrylate.
Claims10
A method of producing acrylate in vivo in a genetically modified bacteria cell comprising
- providing a cell with a first one or more nucleic acids encoding a pathway resulting in the production of 3-hydroxypropionate,
- providing the cell with a second one or more nucleic acids encoding a truncated pcs enzyme lacking a domain converting acrylyl-CoA to propionyl-CoA,
- providing the cell with a third one or more nucleic acids encoding acrylyl-CoA hydrolase,
- wherein the cell expresses the first, second and third one or more nucleic acids and produces acrylate.
A method of genetically modifying a bacteria cell comprising
- providing a cell with a first one or more foreign or exogenous nucleic acids encoding a pathway resulting in the production of 3-hydroxypropionate,
- providing the cell with a second one or more foreign or exogenous nucleic acids encoding a truncated pcs enzyme lacking a domain converting acrylyl-CoA to propionyl-CoA, and
- providing the cell with a third one or more foreign or exogenous nucleic acids encoding acrylyl-CoA hydrolase.
A genetically modifying a bacteria cell comprising
- a first one or more foreign or exogenous nucleic acids encoding a pathway resulting in the production of 3-hydroxypropionate,
- a second one or more foreign or exogenous nucleic acids encoding a truncated pcs enzyme lacking a domain converting acrylyl-CoA to propionyl-CoA, and
- a third one or more foreign or exogenous nucleic acids encoding acrylyl-CoA hydrolase.
Dependencies are inferred from the claim text.