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AssigneeInvista North America S.A.R.L.
InventorsAlexander Van Eck CONRADIE, Adriana L. BOTES, Alec FOSTER, Changlin CHEN
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This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a CoA ligase, an isomerase, a reductase, a thioesterase, a monooxygenase, a hydratase, and/or a thiolase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.
A method of producing non-3-enal and 9-oxononanoate in a recombinant microorganism, said method comprising enzymatically converting 9-hydroxyperoxyoctadec-10,12-dienoate to non-3-enal and 9-oxononanoate using an exogenous polypeptide having the activity of a hydroperoxide lyase classified under EC 4.2.99.-,
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The method of claim 65, wherein said 9-hydroxyperoxyoctadec-10,12-dienoate is enzymatically produced from octadecanoyl-CoA using one or more polypeptides having the activity of a delta9-desaturase, a delta12-desaturase, a thioesterase, and/or a 9-lipoxygenase.
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A recombinant microorganism producing azelaic acid, said microorganism comprising one or more nucleic acids encoding a polypeptide having the enzymatic activities of any one or more of the following enzymes:
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A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having carboxylate reductase activity or a polynucleotide encoding a polypeptide having ω-transaminase activity, wherein the polynucleotide encoding a polypeptide having carboxylate reductase activity is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having carboxylate reductase activity is selected from:
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Means for producing pimeloyl-CoA, comprising culturing a non-naturally occurring microorganism comprising at least one exogenous nucleic acid encoding a polypeptide having the enzymatic activity of (i) a hydroperoxide lyase, (ii) an aldehyde dehydrogenase, (iii) a CoA ligase, (iv) a dodecenoyl-CoA isomerase, (iv) a trans-2-enoyl-CoA reductase, (v) a thioesterase, (vi) an enoate reductase, (vii) a monooxygenase, (viii) an alcohol dehydrogenase, (ix) an acyl-CoA dehydrogenase, (x) an enoyl-CoA hydratase, (xi) a 3-hydroxyacyl-CoA dehydrogenase and (xii) a β-ketothiolase, expressed in a sufficient amount in said microorganism to produce pimeloyl-CoA.
Dependencies are inferred from the claim text.