Method for culturing pluripotent stem cells

A method for culturing induced pluripotent stem cells (iPS cells), the method comprising:

• culturing isolated iPS cells in a pseudo-microgravity environment to proliferate the iPS cells while maintaining the iPS cells in an undifferentiated state, thereby forming and growing spheroids of the iPS cells;
• and performing one cycle of the steps of disrupting the resulting spheroids by passing the spheroid through a filter having a filter mesh size of 40 to 100 μm and culturing the disrupted spheroids in a pseudo-microgravity environment, thereby forming and growing spheroids, or repeating the cycle two or more times.