Enzymatic synthesis of soluble glucan fiber
AssigneeE. I. Du Pont de Nemours and Company
InventorsQiong Cheng, Robert Dicosimo, Arthur Ouwehand, Zheng You, Mark S. Payne, Jian Ping Lai, Kristin Ruebling-Jass, Steven Cary Rothman
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Abstract
An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
Claims13
A soluble α-glucan fiber composition, said soluble α-glucan fiber composition comprising:
- a. a range of:
- i. 0% to 50% of α-(1,3) glycosidic linkages;
- or
- ii. 0% to 40% α-(1,4) glycosidic linkages;
- or
- iii. any combination of i) and ii);
- b. 1 to 50% of a combination of α-(1,2) and α-(1,2,6) glycosidic linkages;
- c. 0-25% α-(1,3,6) glycosidic linkages;
- d. less than 99% α-(1,6) glycosidic linkages;
- e. a weight average molecular weight of less than 300 kDa;
- f. a viscosity of less than 0.25 Pascal second (Pa·s) at 12 wt % in water at 20° C.;
- g. a digestibility of less than 20% as measured by the Association of Analytical Communities (AOAC) method 2009.01;
- h. a solubility of at least 20% (w/w) in pH 7 water at 25° C.;
- and
- i. a polydispersity index of less than 26.
A method to produce an α-glucan fiber composition comprising:
- a. providing a set of reaction components comprising:
- i. sucrose;
- ii. an α-glucan substrate having a weight average molecular weight of at least 0.5 kDa, said α-glucan substrate comprising at least 50% α-(1,6) glycosidic linkages;
- iii. a polypeptide comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 6;
- said polypeptide capable of catalyzing the synthesis of α-(1,2) glycosidic linkages on the α-glucan substrate;
- and
- iv. optionally one or more acceptors;
- b. combining the set of reaction components under suitable aqueous reaction conditions whereby the polypeptide catalyzes the synthesis of an α-glucan fiber composition comprising 1 to 50% α-(1,2) glycosidic linkages;
- and
- c. optionally isolating the α-glucan fiber composition.
A method to produce an α-glucan fiber composition comprising:
- a. contacting sucrose with at least one glucosyltransferase or a combination of at least one glucosyltransferase and at least one α-glucanohydrolase under suitable reaction conditions whereby an α-glucan substrate is produced having a weight average molecular weight of at least 0.5 kDa, said α-glucan substrate comprising at least 50% α-(1,6) glycosidic linkages;
- wherein said α-glucan substrate comprises less than 1% α-(1,2) glycosidic linkages;
- b. contacting the α-glucan substrate produced in (a) with a set of reaction components comprising
- i. a polypeptide comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 6;
- said polypeptide capable of catalyzing the synthesis of α-(1,2) glycosidic linkages on the α-glucan substrate;
- ii. sucrose;
- and
- iii. optionally one or more acceptors;
- c. combining the set of reaction components under suitable aqueous reaction conditions whereby the polypeptide catalyzes the synthesis of an α-glucan fiber composition comprising 1 to 50% α-(1,2) glycosidic linkages;
- and
- d. optionally isolating the α-glucan fiber composition of step (c).
Dependencies are inferred from the claim text.