Biosynthetic production of acetaminophen, p-aminophenol, and p-aminobenzoic acid
OwnerJohn Christopher Anderson, Saurabh Srivastava +3inventors
InventorsJohn Christopher Anderson, Saurabh Srivastava, Mark T. Daly, Patrick Poon, Timothy Revak
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Abstract
The present disclosure provides compositions and methods for the biosynthetic production of acetaminophen, p-aminophenol, and p-aminobenzoic acid and the purification of biologically derived acetaminophen.
Claims18
A non-naturally occurring microbial organism comprising at least three exogenous genes encoding acetaminophen pathway enzymes expressed in a sufficient amount to produce acetaminophen, wherein said acetaminophen pathway comprises (i) an enzyme that converts chorismic acid to p-aminobenzoic acid (ii) an enzyme that converts p-aminobenzoic acid to p-aminophenol and (iii) an enzyme that converts p-aminophenol to acetaminophen.
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A method for producing acetaminophen comprising:
- a. providing a fermentation media comprising carbon substrate;
- and
- b. contacting said media with a recombinant yeast microorganism expressing an engineered acetaminophen biosynthetic pathway wherein said pathway comprises the following substrate to product conversions;
- i. chorismic acid to p-aminobenzoic acid (PABA) (pathway step a);
- ii. p-aminobenzoic acid to p-aminophenol (pathway step b);
- iii. p-aminophenol to acetaminophen (pathway step c);
- and
- c. culturing the yeast in conditions whereby acetaminophen is produced.
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A method for purifying acetaminophen comprising
- (a) filtering a liquid sample that comprises biologically derived acetaminophen with a reverse osmosis filter to produce a retentate;
- (b) heating the retentate to 80° C. to evaporate liquid;
- (c) cooling the remaining solution;
- (d) filtering the solution;
- (e) collecting the acetaminophen crystals;
- and
- (f) drying the crystals to obtain purified acetaminophen.
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-50. (canceled)
Dependencies are inferred from the claim text.