Biosynthetic production of choline, ethanolamine, phosphoethanolamine, and phosphocholine
OwnerJohn Christopher Anderson, Saurabh Srivastava +1inventors
InventorsJohn Christopher Anderson, Saurabh Srivastava, Mark T. Daly
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Abstract
The present disclosure provides compositions and methods for the biosynthetic production of choline, ethanolamine, phosphoethanolamine, and phosphocholine.
Claims14
A modified microorganism for production of choline comprising at least three heterologous enzymes selected from the group consisting of serine decarboxylase, ethanolamine kinase, phosphoethanolamine N-methyltransferase, and phosphocholine phosphatase.
A non-naturally occurring microbial organism comprising at least three heterologous genes encoding choline pathway enzymes expressed in a sufficient amount to produce choline, wherein said choline pathway comprises (i) an enzyme that converts serine to ethanolamine, (ii) an enzyme that converts ethanolamine to phosphoethanolamine, (iii) enzyme that converts phosphoethanolamine to phosphocholine, and (iv) an enzyme that converts phosphocholine to choline.
-29. (canceled)
Dependencies are inferred from the claim text.