Enzymatic synthesis of soluble glucan fiber
AssigneeE. I. Du Pont de Nemours and Company
InventorsRobert DICOSIMO, Qiong CHENG, Andrew C. ELIOT, Arthur OUWEHAND, Mark S. PAYNE, Zheng YOU
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Abstract
An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
Claims14
A soluble α-glucan fiber composition, said soluble α-glucan fiber composition comprising:
- a. 25-35% α-(1,3) glycosidic linkages;
- b. 55-75% α-(1,6) glycosidic linkages;
- c. 5-15% α-(1,3,6) glycosidic linkages;
- d. a weight average molecular weight of less than 5000 Daltons;
- e. a viscosity of less than 0.25 Pascal second (Pa·s) at 12 wt % in water at 20° C.;
- f. a dextrose equivalence (DE) in the range of 4 to 40;
- and
- g. a digestibility of less than 12% as measured by the Association of Analytical Communities (AOAC) method 2009.01;
- h. a solubility of at least 20% (w/w) in water at 25° C.;
- and
- i. a polydispersity index of less than 5.
A method to produce a soluble α-glucan fiber composition comprising:
- a. providing a set of reaction components comprising:
- i. sucrose;
- ii. at least one polypeptide having glucosyltransferase activity;
- said polypeptide comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 10 or 12;
- iii. at least one polypeptide having α-glucanohydrolase activity;
- said polypeptide selected from the group of:
- 1. a polypeptide having mutanase activity, said polypeptide comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 1, 3, 6 or 8;
- 2. a polypeptide comprising dextranase activity;
- and
- 3. a combination of 1 and 2.;
- and
- iv. optionally one or more acceptors;
- b. combining the set of reaction components under suitable aqueous reaction conditions whereby a product comprising a soluble α-glucan fiber composition is produced;
- and
- c. optionally isolating the soluble α-glucan fiber composition from the product of step (b).
Dependencies are inferred from the claim text.