Method for reconstructing a non-human animal embryo
AssigneeUniversita' Degli Studi Di Teramo
InventorsPasqualino LOI, Domenico IUSO, Marta CZERNIK, Federica ZACCHINI, Grazyna PTAK, Saadi KHOCHBIN, Antonella FIDANZA
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Abstract
The present invention refers to a method for reconstructing a non-human animal embryo, to a method for generating a nonhuman animal and to a non-human animal or non-human animal reconstructed embryo obtainable by the methods according to the invention. The method of somatic cell nuclear transfer (SCNT) has a step of transiently expressing or inducing the uptake of a protein involved in male sperm maturation, such as protamine or transition protein, in the somatic donor cell before nuclear transfer.
Claims15
A method for reconstructing a non-human animal embryo, comprising:
- a) transiently expressing into a non-human donor cultivated cell a protein involved in male sperm maturation or a synthetic or recombinant portion thereof able to confer to the chromatin of said cell a spermatozoa like chromatin conformation;
- or
- a′) inducing the uptake from the cultivating medium of a non-human donor cultivated cell of at least a protein involved in male sperm maturation or a synthetic or recombinant portion thereof able to confer to the chromatin of said cell a spermatozoa like chromatin conformation,
- b) identifying the nuclei wherein the chromatin has a spermatozoa like chromatin conformation;
- c) transplanting the identified nuclei into a non-human animal enucleated oocyte to get reconstructed embryo and optionally;
- and
- d) culturing said reconstructed embryo in vitro or in vivo.
A method for generating a non-human animal, comprising:
- a) transferring a reconstructed non-human animal embryo into a suitable non-human recipient animal or intermediate host or eggshell;
- b) causing said non-human animal embryo to develop to term;
- and
- c) further breeding the resulting non-human animal.
Dependencies are inferred from the claim text.