Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme b synthesis
AssigneeINVISTA North American S.á r.I.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Abstract
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.
Claims7
-32. (canceled)
A recombinant host comprising at least one exogenous nucleic acid encoding (i) a (homo)ncitrate synthase classified under EC 2.3.3.14 or EC 2.3.3.13, (ii) a (homo)ncitrate dehydratase and a (homo)naconitate hydratase classified under EC 4.2.1.114, EC 4.2.1.36, or EC 4.2.1.33, (iii) an iso(homo)ncitrate dehydrogenase classified under EC 1.1.1.85, EC 1.1.1.87, or EC 1.1.1.286, and/or (iv) an indolepyruvate decarboxylase classified under EC 4.1.1.43 or EC 4.1.1.74, or a 2-oxoglutarate dehydrogenase complex comprising one or more enzymes classified under EC 1.2.4.2, EC 1.8.1.4, and EC 2.3.1.61, said host producing pimeloyl-CoA or pimelate semialdehyde.
Dependencies are inferred from the claim text.