Milk clotting aspartic protease enzyme composition
AssigneeChr. Hansen A/S
InventorsMARTIN LUND, Enikoe Fodor HANSEN, Jonas JACOBSEN, Johannes Maarten VAN DEN BRINK
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Abstract
A liquid or dried granulated milk clotting aspartic protease enzyme composition comprising added polypeptides/proteins. The polypeptides/proteins may be animal-derived (e.g. whey, lactalbumin, transferrin, casein, ovalbumin, gelatin, blood), vegetable-derived (soy, pea, corn, potato, hemp, rice, wheat, peanut, sun flower, rape seed) or algae proteins (e.g. spirulina). Addition of protein in several instances increases activity of the enzyme and simultaneously improves physical stability.
Claims21
1-16. (canceled)
A method for making a liquid milk clotting aspartic protease enzyme composition, comprising:
- obtaining a liquid milk clotting aspartic protease enzyme sample having a milk clotting aspartic protease enzyme strength of from 25 IMCU/g to 30,000 IMCU/g, wherein milk clotting aspartic protease enzyme constitutes at least 70% by weight of the total amount of proteins present in the sample having a size bigger than 10 kDa as determined by SDS-PAGE;
- and
- adding to the sample an amount of a polypeptide formulation comprising polypeptides longer than 10 amino acid residues, wherein the polypeptides are not milk clotting aspartic protease enzymes and are not enzymes that degrade the milk clotting aspartic protease enzymes,
- wherein the amount of added polypeptide formulation results in a concentration of said polypeptides in the composition of from 0.01% to 10% (w/w) and is effective to obtain a liquid milk clotting aspartic protease enzyme composition having a milk clotting aspartic protease enzyme strength of from 26 IMCU/g to 30,100 IMCU/g of the composition, wherein the milk clotting aspartic protease enzyme strength of the composition is at least 1% higher than the milk clotting aspartic protease enzyme strength of the sample when measured after storage for one week at 5° C.
A liquid milk clotting aspartic protease enzyme composition, comprising:
- (a) milk clotting aspartic protease enzyme at a strength of from 25 IMCU/g to 30,000 IMCU/g of the composition;
- (b) polypeptides longer than 10 amino acid residues at a concentration from 0.01% to 10% (w/w) of the composition, wherein the polypeptides are not milk clotting aspartic protease enzymes and comprise one or more selected from whey proteins, alpha lactalbumin, beta-lactoglobulin, transferrin, lactoperoxidase, casein, alpha-s1-casein, alpha-s2-casein, beta-casein, kappa-casein, ovalbumin, gelatin, bovine serum albumin, soy proteins, pea proteins, corn proteins, potato proteins, hemp proteins, rice proteins, spirulina proteins, wheat proteins, peanut proteins, sun flower proteins, rape seed proteins, blood proteins, and algae proteins;
- and
- (c) a salt in a concentration of from 1 to 350 g/kg,
- wherein the pH of the composition is from 2 to 8.
A dried granulated milk clotting aspartic protease enzyme composition comprising:
- (a) milk clotting aspartic protease enzyme at a strength of from 25 IMCU/g to 30,000 IMCU/g of the composition;
- (b) polypeptides longer than 10 amino acid residues at a concentration from 0.01% to 10% (w/w) of the composition, wherein the polypeptides are not milk clotting aspartic protease enzymes and comprise one or more selected from whey proteins, alpha lactalbumin, beta-lactoglobulin, transferrin, lactoperoxidase, casein, alpha-s1-casein, alpha-s2-casein, beta-casein, kappa-casein, ovalbumin, gelatin, bovine serum albumin, soy proteins, pea proteins, corn proteins, potato proteins, hemp proteins, rice proteins, spirulina proteins, wheat proteins, peanut proteins, sun flower proteins, rape seed proteins, blood proteins, and algae proteins;
- and
- (c) a salt,
- wherein the pH of the composition when suspended in water is from 2 to 8.
Dependencies are inferred from the claim text.