Device and methods of using device for detection of aminoacidopathies
AssigneeUniversity of Maryland, College Park
InventorsOmar Bilal AYYUB, Brian Theodore HELIGMAN, Peter KOFINAS, Marshall Lynn SUMMAR, Juan Manuel CABRERA-LUQUE, Gary Cunningham
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Abstract
The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.
Claims40
A biosensor comprising:
- a light source;
- a light detector;
- and
- at least one reaction surface comprising at least one electron mediator, at least one reduction agent, and at least one metabolic enzyme or functional fragment thereof;
- wherein the reaction surface does not comprise and is not attached to an electrically conductive support;
- and
- wherein the light source is positioned at a distance from the reaction surface sufficient to irradiate the reaction surface and the light detector is positioned at a distance from the reaction surface sufficient to collect irradiated light from the reaction surface.
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A biosensor comprising:
- at least one reaction surface comprising at least one electron mediator, at least one reduction agent, and at least one metabolic enzyme or functional fragment thereof;
- wherein the at least one enzyme or functional fragment thereof is at least 70% homologous to a phenylalanine dehydrogenase from Geobacillus thermoglucosidiasus;
- and
- wherein the reaction surface does not comprise and is not attached to an electrically conductive support.
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A test strip comprising at least one reaction surface, wherein the reaction surface comprises at least one reduction agent and at least one metabolic enzyme or functional fragment thereof, wherein the reaction surface does not comprise and is not attached to an electrode or an electrically conductive support.
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Dependencies are inferred from the claim text.