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US 2017/0198323 A1Published application
Enzymatic synthesis of soluble glucan fiber
AssigneeE. I. Du Pont de Nemours and Company
InventorsQiong Cheng, Robert Dicosimo, Arthur Ouwehand, Zheng You, Mark S. Payne, Jahnavi Chandra Prasad
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Abstract
An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
Claims15
A soluble α-glucan fiber composition comprising:
- a. 10-30% α-(1,3) glycosidic linkages;
- b. 65-87% α-(1,6) glycosidic linkages;
- c. less than 5% α-(1,3,6) glycosidic linkages;
- d. a weight average molecular weight of less than 5000 Daltons;
- e. a viscosity of less than 0.25 Pascal second (Pa·s) at 12 wt % in water at 20° C.;
- f. a dextrose equivalence (DE) in the range of 4 to 40;
- and
- g. a digestibility of less than 12% as measured by the Association of Analytical Communities (AOAC) method 2009.01;
- h. a solubility of at least 20% (w/w) in pH 7 water at 25° C.;
- and
- i. a polydispersity index of less than 5.
8 more elements
A method to produce a soluble α-glucan fiber composition comprising:
- a. providing a set of reaction components comprising:
- i. sucrose;
- ii. at least one polypeptide having glucosyltransferase activity, said polypeptide comprising an amino acid sequence having at least 90% identity to a sequence selected from SEQ ID NOs:
- 1 and 3;
- iii. at least one polypeptide having α-glucanohydrolase activity;
- and
- iv. optionally one or more acceptors;
- b. combining the set of reaction components under suitable aqueous reaction conditions whereby a product comprising a soluble α-glucan fiber composition is produced;
- and
- c. optionally isolating the soluble α-glucan fiber composition from the product of step (b).
7 more elements
Dependencies are inferred from the claim text.