Method of producing l-amino acids

A method of producing L-amino acids, the method comprising

• 1) culturing a recombinant coryneform microorganism capable of producing L-amino acids in a medium, wherein the recombinant coryneform microorganism is transformed by inserting an acetate-inducible promoter downstream of a stop codon of a target gene in a chromosome such that transcription from the acetate-inducible promoter occurs in a direction opposite to the direction of the target gene transcription, wherein the target gene is a gene located at a branch point of a biosynthetic pathway of an L-amino acid;
• and
• 2) adding acetate during the culturing to cause an inverse transcription of the target gene from the acetate-inducible promoter so that RNA polymerase complexes conflict with each other to weaken the expression of the target gene, and to strengthen the L-amino acid production capability of the recombinant coryneform microorganism.