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US 2017/0192012 A1Published application
Compositions and methods for detecting s-nitrosylation and s-sulfinylation
AssigneeThe Regents of the University of Michigan
InventorsBrent R. Martin, Jaimeen D. Majmudar
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Abstract
The present invention relates to methods for detecting protein S-sulfinylation and S-sulfinylation within thiol groups in proteins, metabolites, or materials.
Claims26
1-21. (canceled)
A method for detecting protein S-nitrosylation within cysteine residues of a protein, comprising:
- providing a biological sample comprising one or more proteins having cysteine residues and a composition comprising a sulfinic acid moiety,
- exposing the composition to the biological sample such that an interaction between the sulfinic acid moiety and a S-nitrosothiol side chain of a cysteine residue results in generation of a labeled sulfonothioate moiety, and
- characterizing the cysteine residues of the one or more proteins having sulfonothioate moieties as having undergone S-nitrosylation.
A method for detecting protein S-nitrosylation within cysteine residues of a protein, comprising:
- providing a biological sample comprising one or more proteins having cysteine residues and a composition comprising a nitrosothiol moiety,
- exposing the composition to the biological sample such that an interaction between the nitrosothiol moiety and a sulfinic acid side chain of a cysteine residue results in generation of a sulfonothioate moiety, and
- characterizing the cysteine residues of the one or more proteins having sulfonothioate moieties as having undergone S-nitrosylation.
Dependencies are inferred from the claim text.