Method for the enzymatic production of d-erythrose and acetyl phosphate

An organism or microorganism, which expresses

• (i) a phosphoketolase;
• and
• (ii) a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13)
• and produces acetyl phosphate from D-fructose.

A composition comprising

• (i) D-fructose;
• and
• (ii) a phosphoketolase;
• and optionally
• (iii) a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13)
• or
• (i) D-erythrose;
• and
• (ii) a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13);
• and optionally
• (iii) a phosphoketolase or a sulfoacetaldehyde acetyltransferase (EC 2.3.3.15);
• or
• (i) D-glucose;
• (ii) a glucose-fructose isomerase;
• and
• (iii) a phosphoketolase;
• and optionally
• (iv) a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13).

A composition comprising

• (i) D-fructose;
• and
• (ii) an organism or microorganism expressing a phosphoketolase or expressing a phosphoketolase and a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or expressing a phosphoketolase and a fructose-bisphosphate aldolase (EC 4.1.2.13);
• or
• (i) D-erythrose;
• and
• (ii) an organism or microorganism expressing a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or expressing a fructose-bisphosphate aldolase (EC 4.1.2.13) or expressing a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) and a phosphoketolase or a sulfoacetaldehyde acetyltransferase (EC 2.3.3.15) or expressing a fructose-bisphosphate aldolase (EC 4.1.2.13) and a phosphoketolase or a sulfoacetaldehyde acetyltransferase (EC 2.3.3.15);
• or
• (i) D-glucose;
• and
• (iii) an organism or microorganism expressing a glucose-fructose isomerase and expressing a phosphoketolase and, optionally, expressing a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13).