Oligosaccharide production
AssigneeGlycom A/S
InventorsEric Samain, Pauline Peltier-Pain, Katrine Bych, Ted Johanson, Elise Champion, Gyula Dekany
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Abstract
The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source. The one or more genes encoding a sucrose utilization system are preferably one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, such as the scr genes.
Claims22
A process for making a recombinant oligosaccharide or a glycoside of said oligosaccharide by glycosylating a carbohydrate acceptor, which is a monosaccharide or disaccharide and which is not sucrose, or a glycoside of said acceptor, comprising the steps of:
- a) providing a cell that can internalize said acceptor into said cell and comprises
- a recombinant gene encoding a glycosyl transferase which is able to transfer a glycosyl residue of an activated sugar nucleotide to said acceptor, internalized in said cell, and
- a biosynthetic pathway to make said activated sugar nucleotide in said cell,
- b) culturing said cell in the presence of said acceptor and sucrose, and
- c) separating said oligosaccharide from said cell, from the culture medium or from both, said process being characterized in that said cell also comprises one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, so that said cell can use sucrose as a carbon source for making said activated sugar nucleotide and as an energy source for making said oligosaccharide.
A genetically modified microorganism for making a recombinant oligosaccharide or a glycoside of said oligosaccharide, comprising:
- a recombinant gene encoding a glycosyl transferase which can transfer a glycosyl residue of an activated sugar nucleotide to a carbohydrate acceptor, which is a monosaccharide or a disaccharide and which is not sucrose, or a glycoside of said acceptor internalized by the microorganism,
- a biosynthetic pathway for making said activated sugar nucleotide from sucrose, and
- one or more genes encoding a heterologous PTS-dependent sucrose utilization system so that said cell is capable to use sucrose as a carbon source for making said activated sugar nucleotide, and as an energy source.
Dependencies are inferred from the claim text.