Apparatus and Method for Culturing Cells and Tissue
AssigneeIonoptix LLC
InventorsPeter G.W. LEE, Douglas L. TILLOTSON, Richard T. UDALE, Katherine L. BARBER
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Abstract
A method of culturing cells or tissue comprises: (a) disposing cells or tissue in the cavity of a deformable cell culture container; and (b) applying synchronized chronic electrical stimulation and stretch to the cells or tissue in the deformable culture cell container, wherein the synchronized chronic electrical stimulation is applied in the form of pulses at a frequency of from 0.010-99 Hz and a duration of from 0.4 to 24 ms; and wherein stretch cycles are applied to the cells or tissue at a frequency of from 0.010 to 15 Hz.
Claims11
A method of culturing cells or tissue comprising:
- (a) disposing cells or tissue in the cavity of a deformable cell culture container;
- and
- (b) applying synchronized chronic electrical stimulation and stretch to the cells or tissue in the deformable culture cell container,
- wherein the synchronized chronic electrical stimulation is applied in the form of pulses at a frequency of from 0.010-99 Hz and a duration of from 0.4 to 24 ms;
- and
- wherein stretch cycles are applied to the cells or tissue at a frequency of from 0.010 to 15 Hz.
Dependencies are inferred from the claim text.