Novel (r)-hydroxynitrile lyase
AssigneePublic University Corporation Toyama Prefectural University
InventorsYasuhisa ASANO, Mohammad Lakmehsari DADASHIPOUR, Yuko ISHIDA
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Abstract
The objective of the present invention is to provide a (R)-hydroxynitrile lyase which is more stable than a hydroxynitrile lyase derived from a plant, a gene which encodes the hydroxynitrile lyase and by which heterologous expression is possible, and a method for producing the hydroxynitrile lyase. The (R)-hydroxynitrile lyase according to the present invention is characterized in having the specific amino acid sequence such as the amino acid sequence of SEQ ID NO: 3.
Claims14
A (R)-hydroxynitrile lyase having any one of the following amino acid sequences (1) to (3):
- (1) an amino acid sequence of SEQ ID NO:
- 3;
- (2) an amino acid sequence specified in the (1) with deletion, substitution and/or addition of 1 or more amino acid residues, wherein a hydroxynitrile lyase activity of a (R)-hydroxynitrile lyase having the amino acid sequence (2) is similar to or superior to that of natural (R)-hydroxynitrile lyase having the amino acid sequence of SEQ ID NO:
- 3;
- (3) an amino acid sequence having at least 30% sequence homology to the amino acid sequence specified in the (1), wherein a hydroxynitrile lyase activity of a (R)-hydroxynitrile lyase having the amino acid sequence (3) is similar to or superior to that of natural (R)-hydroxynitrile lyase having the amino acid sequence of SEQ ID NO:
- 3.
A (R)-hydroxynitrile lyase gene having any one of the following base sequences (4) to (6):
- (4) a base sequence of SEQ ID NO:
- 2;
- (5) a base sequence specified in the (4) with deletion, substitution and/or addition of 1 or more bases, wherein a hydroxynitrile lyase activity of a (R)-hydroxynitrile lyase encoded by the gene having the base sequence (5) is similar to or superior to that of natural (R)-hydroxynitrile lyase encoded by the gene having the base sequence of SEQ ID NO:
- 2;
- (6) a base sequence having at least 30% sequence homology to the base sequence specified in the (4), wherein a hydroxynitrile lyase activity of a (R)-hydroxynitrile lyase encoded by the gene having the base sequence (6) is similar to or superior to that of natural (R)-hydroxynitrile lyase encoded by the gene having the base sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.