Bacterial cytochrome p450 protein variant and method of reducing concentration of fluorinated methane in sample using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsYukyung Jung, Dongsik Yang, Jinhwan Park, Taeyong Kim, Changduk Kang, Anirban Bhaduri, Tadi Venkata Siva Kumar
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Abstract
Provided is a recombinant microorganism including an exogenous gene encoding a bacterial cytochrome P450 protein or a variant thereof, a composition including the recombinant P450 protein or the variant thereof, which is used for removing CHnF4-n (n is an integer of 0 to 3) in a sample, and a method of reducing a concentration of CHnF4-n in the sample.
Claims17
A recombinant microorganism comprising an exogenous gene encoding a bacterial cytochrome P450 protein or a variant thereof, wherein the variant is a P450Cam variant having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 and having an activity belonging to EC 1.14.15.1, or wherein the variant is a P450BM3 variant having an amino acid alteration at an amino acid residue corresponding to position N320 of SEQ ID NO:
- 8 and having an activity belonging to EC 1.14.14.1.
A composition comprising a recombinant P450 protein variant, wherein the variant is a P450Cam variant having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 and having an activity belonging to EC 1.14.15.1, or a P450BM3 variant having an amino acid alteration at an amino acid residue corresponding to position N320 of SEQ ID NO:
- 8 and having an activity belonging to EC 1.14.14.1.
A method of reducing a concentration of fluorinated methane in a sample, the method comprising contacting a recombinant P450 protein or a variant thereof with the sample comprising fluorinated methane represented by CHnF4-n (n is an integer of 0 to 3) to reduce the concentration of fluorinated methane in the sample, wherein the variant is a P450Cam variant having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 and having an activity belonging to EC 1.14.15.1, or a P450BM3 variant having an amino acid alteration at an amino acid residue corresponding to position N320 of SEQ ID NO:
- 8 and having an activity belonging to EC 1.14.14.1.
A variant bacterial P450Cam polypeptide having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 and belonging to EC 1.14.15.1, or a variant bacterial P450BM3 polypeptide having an amino acid alteration at an amino acid residue corresponding to position N320 of SEQ ID NO:
- 8 and belonging to EC 1.14.14.1.
A polynucleotide encoding the variant of bacterial P450Cam or the variant of bacterial P450BM3 of claim 17.
Dependencies are inferred from the claim text.