Reduction of oxidated methionine peptides for mass spectrometry
AssigneeCalifornia Institute of Technology
InventorsMichael Sweredoski, Annie Moradian, Tanya R. Yakushi, Sonja Hess, Roxana Eggleston-Rangel
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Abstract
A method for preparing a methionine-containing peptide or protein for analysis by mass spectrometry includes incubating the methionine-containing peptide or protein with a heterologous methionine sulfoxide reductase A (MsrA), MsrB, or MsrAB enzyme resulting in the reduction of oxidized methionine-containing peptides or proteins.
Claims20
A method for preparing a methionine-containing peptide or protein for analysis by mass spectrometry, comprising:
- incubating the methionine-containing peptide or protein with a heterologous methionine sulfoxide reductase (Msr) enzyme.
A method for preparing a methionine-containing peptide or protein for analysis by mass spectrometry, comprising:
- immobilizing a heterologous Msr enzyme on a resin bead or a membrane filter;
- activating the immobilized heterologous Msr enzyme;
- adding the methionine-containing peptide or protein to the heterologous Msr enzyme;
- and
- separating the methionine-containing peptide or protein from the immobilized heterologous Msr enzyme after incubation.
A kit for reducing a methionine-containing peptide or protein, the kit comprising a heterologous methionine sulfoxide reductase (Msr) enzyme.
Dependencies are inferred from the claim text.