Method for producing polymers
AssigneeSynthetic Genomics, Inc.
InventorsPeer F. Staehler, Cord F. Staehler, Manfred Mueller
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps: (a) provision of a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesis of nucleic acid fragments having in each case different base sequences in several of the individual reaction areas, and (c) detachment of the nucleic acid fragments from individual reaction areas.
Claims33
Method for directly converting digital target sequences into nucleic acids comprising the steps
- (a) providing a digital target sequence,
- (b) fragmenting the digital target sequence into suitable oligomeric building blocks,
- (c) synthesizing said oligomeric building blocks on at least one support by parallel synthesis steps,
- (d) detaching the oligomeric building blocks from the support,
- (e) bringing the oligomeric building blocks into contact with one another, and
- (f) linking the phosphodiester backbone enzymatically.
Method for generating functionally integrated DNA molecules, comprising the steps
- (a) providing digital nucleic acid sequences of several functional elements,
- (b) integrating the functional elements into a digital DNA molecule,
- (c) fragmenting the digital DNA molecule into suitable oligomeric building blocks,
- (d) synthesizing said oligomeric building blocks on at least one support by parallel synthesis steps,
- (e) detaching the oligomeric building blocks from the support,
- (f) bringing the oligomeric building blocks into contact with one another, and
- (g) linking the phosphodiester backbone enzymatically to form the integrated DNA molecule.
Dependencies are inferred from the claim text.