Cell-Based Production of Nonulosonates
AssigneesNational Research Council of Canada, University of Ottawa
InventorsDennis M. WHITFIELD, Susan M. LOGAN, Ian C. SCHOENHOFEN, Christopher N. BODDY, Benjamin R. LUNDGREN
Life of this patent
Estimating the term…
Abstract
The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose. Methods for producing the sugars are also provided.
Claims19
A recombinant cell for the production of legionaminic acid, comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding a PglF enzyme function, a gene encoding a PglE enzyme function, a gene encoding a PglD enzyme function, a gene encoding a LegI enzyme function, a gene encoding a LegG enzyme function, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
A recombinant cell for the production of UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose (UDP-BacdiNAc), comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding a PglF enzyme function, a gene encoding a PglE enzyme function, a gene encoding a PglD enzyme function, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
Dependencies are inferred from the claim text.