Device and method of collection for rna viruses
AssigneeGeneral Electric Company
InventorsErik Leeming Kvam, Robert Scott Duthie, John Richard Nelson
Life of this patent
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Abstract
The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
Claims30
A method of inactivation and dry storage of a biological sample containing an RNA virus, the method comprising:
- providing a non-dissolvable, dry, solid matrix incorporated with a composition, the composition comprising at least one protein denaturant, at least one acid or acid-titrated buffer reagent impregnated therein in a dry state;
- contacting the biological sample with the dry solid matrix to effectively lyse cells of the biological sample and preserve the RNA in an intact state;
- drying the biological sample on the solid matrix;
- and
- storing the biological sample on the solid matrix in a dry state under ambient conditions wherein the sample is rendered not infectious and the RNA therein is maintained in an intact state.
A method for testing a biological sample for the presence of an RNA virus, the method comprising:
- providing a non-dissolvable, dry, solid matrix incorporated with a composition, the composition comprising at least one protein denaturant, at least one acid or acid-titrated buffer reagent impregnated therein in a dry state;
- applying the biological sample to the dry solid matrix;
- drying the biological sample on the solid matrix;
- recovering from the dry solid matrix RNA in the biological sample;
- and
- analyzing the recovered RNA for the presence of viral RNA.
A collection device for inactivation and dry storage of a biological sample containing an RNA virus without degradation of viral RNA, the device comprising:
- providing a non-dissolvable, dry, solid matrix incorporated with a composition, the composition comprising at least one protein denaturant, at least one acid or acid-titrated buffer reagent impregnated therein in a dry state;
- a frame for holding the dry solid matrix;
- and
- a storage sleeve capable of encasing the frame and dry solid matrix after collection of the sample.
Dependencies are inferred from the claim text.