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US 2017/0138945 A1Published application
Method for measuring endotoxin
AssigneeSeikagaku Corporation
InventorsHikaru Mizumura, Maki Aizawa, Toshio Oda
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Abstract
A method for rapidly and highly sensitively measuring endotoxin is provided. Endotoxin is measured using an endotoxin-measuring agent comprising the proteins (1) to (3) below, each of which is a recombinant protein obtainable by being expressed using insect cells as a host: (1) a factor C derived from Tachypleus tridentatus, which factor C does not have a His-tag sequence at the C-terminus; (2) a factor B of a horseshoe crab; and (3) a proclotting enzyme of a horseshoe crab.
Claims5
A method for measuring an endotoxin in a test sample, the method comprising steps (a) and (b) below:
- (a) a step of mixing an endotoxin-measuring agent with the test sample;
- and
- (b) a step of measuring progress of a cascade reaction mediated by proteins (1) to (3) below:
- wherein the endotoxin-measuring agent comprises the proteins (1) to (3) below, each of which is a recombinant protein:
- (1) a horseshoe crab factor C or variant thereof having factor C activity, which factor C does not have His-tag sequence at the C-terminus;
- (2) a horseshoe crab factor B or variant thereof having factor B activity;
- and
- (3) a horseshoe crab proclotting enzyme or variant thereof having proclotting enzyme activity.
5 more elements
Dependencies are inferred from the claim text.