Bacteria engineered for conversion of ethylene to n-butanol
AssigneeThe Regents of the University of California
InventorsShota ATSUMI, Michael D. TONEY, Gabriel M. RODRIGUEZ, Yohei TASHIRO, Justin B. SIEGEL, D. Alexander CARLIN, Irina KORYAKINA, Shuchi H. DESAI
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Abstract
The present disclosure provides recombinant bacteria with elevated production of ethanol and/or n-butanol from ethylene. Methods for the production of the recombinant bacteria, as well as for use thereof for production of ethanol and/or n-butanol are also provided.
Claims27
A bacterium comprising a recombinant polynucleotide encoding an ethylene hydratase (EH), wherein expression of the EH results in an increase in production of ethanol as compared to a corresponding bacterium lacking the recombinant polynucleotide.
A bacterium comprising a recombinant polynucleotide encoding an alkene monooxygenase (AMO) and an ethylene oxide reductase (EOR), wherein expression of the AMO and the EOR results in an increase in production of ethanol as compared to a corresponding bacterium lacking the recombinant polynucleotide.
14-21. (canceled)
A bacterium comprising a recombinant polynucleotide encoding an alkene monooxygenase (AMO) and an epoxide hydrolase (EPH), wherein expression of the AMO and the EPH results in an increase in production of ethylene glycol as compared to a corresponding bacterium lacking the recombinant polynucleotide.
27-33. (canceled)
38-40. (canceled)
Dependencies are inferred from the claim text.