Plant synthesizing hypoallergenic paucimannose type n-glycan and uses thereof

Abstract

A quadruple mutant (qm) plant is deficient of functions of core α1,3-fucosyltransferase A (FucTA), core α1,3-fucosyltransferase B (FucTB), β1,2-xylosyltransferase (XylT), and β1,2-N-acetylglucosaminyltransferase II (GnTII), and produces a protein containing hypoallergenic pauci-mannose-type N-glycan that does not include α1,3-fucose and β1,2-xylose residues. A method for producing a transgenic plant for a production of a protein containing hypoallergenic pauci-mannose-type N-glycan that does not include α1,3-fucose and β1,2-xylose residues but includes α1,6-fucose residue includes preparing the quadruple mutant (qm) plant, and transforming the quadruple mutant plant with a recombinant vector containing a gene encoding the human-derived α1,6-fucosyltransferase (FUT8) protein to overexpress FUT8 gene, and selecting a transgenic plant which is deficient of the functions of FucTA, FucTB, XylT and GnTII proteins.

Dependencies are inferred from the claim text.