Plants with engineered endogenous genes

A method of increasing a starch level in a plant comprising expressing a first nucleic acid encoding a nuclease capable of inducing a double-strand break in a target sequence included in a sequence of an endogenous gene encoding a Glucan Water Dikinase in a plant, and selecting a homozygous plant that includes a mutation in the target sequence and has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type sequence.

A method of preparing animal feed comprising processing a genetically engineered plant comprising a nucleic acid encoding a restricting enzyme capable of cutting a target sequence, wherein the target sequence is in an endogenous gene encoding a Glucan Water Dikinase, wherein upon the expression of the nucleic acid, the genetically engineered plant has an altered level of vegetative starch compared to the level of vegetative starch in a non-genetically engineered plant, and processing includes at least one of operation selected from the group consisting of harvesting, bailing, grinding, milling, chopping, size reducing, crushing, extracting a component from the feedstock, purifying a component or portion of the feedstock, and extracting or purifying starch.