Aptamer inhibiting biological activity of autotaxin by binding with autotaxin, and use thereof
AssigneeRibomic Inc.
InventorsHisako IKEDA, Shin MIYAKAWA
Life of this patent
Estimating the term…
Abstract
The present invention provides an aptamer that binds to an autotaxin, which contains a nucleotide sequence represented by the following formula (I): CGGAACC-N1-GGTC (I) wherein N1 shows any of 3 to 11 nucleotides, and a utilization method thereof.
Claims22
An aptamer that binds to an autotaxin, which comprises a nucleotide sequence represented by the following formula (I):
- CGGAACC-N1-GGTC (I)
- wherein N1 shows any of 3 to 11 nucleotides.
An aptamer that binds to an autotaxin, which comprises a nucleotide sequence represented by the following formula (II):
- X3X1CGGAACC-N1-GGTCX2X4 (II)
- wherein N1 shows any of 7 to 11 nucleotides, X1, X2, X3 and X4 are each any nucleotide, and at least one of X1 and X2, and X3 and X4 forms Watson-Crick base pairs.
An aptamer that binds to an autotaxin, which comprises a nucleotide sequence shown by the following (a), (b) or (c):
- (a) a nucleotide sequence selected from SEQ ID NOs:
- 4-14, 16, 20-25, 27 and 29;
- (b) a nucleotide sequence selected from SEQ ID NOs:
- 4-14, 16, 20-25, 27 and 29, wherein one or several nucleotides other than sequences shown by CGGAACC and GGTC in CGGAACC-N1-GGTC wherein N1 shows any of 3 to 11 nucleotides are substituted, deleted, inserted or added;
- or
- (c) a nucleotide sequence having not less than 70% identity with a nucleotide sequence selected from SEQ ID NOs:
- 4-14, 16, 20-25, 27 and 29 (excluding sequences shown by CGGAACC and GGTC in CGGAACC-N1-GGTC wherein N1 is as defined above).
An aptamer that binds to an autotaxin, which has a potential secondary structure represented by the following formula (IV):
- wherein X1, X2, X3 and X4 are each any nucleotide, and at least one of X1 and X2, and X3 and X4 forms Watson-Crick base pairs.
(canceled)
(canceled)
Dependencies are inferred from the claim text.