Yeast strain and microbial method for production of pentacyclic triterpenes and/or triterpenoids

A modified yeast strain for production of pentacyclic triterpenoids, comprising:

• i. at least one copy of a gene for encoding an oxidosqualene cyclase, wherein the gene comprises a sequence selected from the group consisting of nucleic acids according to accession number AB055511 (SEQ ID No 1), AB025343 (SEQ ID No 2), AB663343 (SEQ ID No 3), NM_179572 (SEQ ID No 4), AB181245 (SEQ ID No 5), DQ268869 (SEQ ID No 6), AB025345 (SEQ ID No 7), AB116228 (SEQ ID No 8), JQ087376 (SEQ ID No 9), HM623871 (SEQ ID No 10), AB289586 (SEQ ID No 11), AB055512 (SEQ ID No 12) and nucleic acid sequence variants with at least 70% sequence identity to SEQ ID No 1 to 12,
• or wherein the gene comprises a sequence which codes for an amino acid sequence according to SEQ ID No 54 to 65, or for an amino acid sequence variant with at least 85% sequence identity to SEQ ID No 54 to 65;
• and/or
• ii. at least one copy of a gene for encoding a NADPH-cytochrome P450 reductase, wherein the gene comprises a sequence selected from the group consisting of nucleic acids according to accession number AB433810 (SEQ ID No 13), X66016 (SEQ ID No 14), X69791 (SEQ ID No 15), XM_003602850 (SEQ ID No 16), NM_001179172 (SEQ ID No 17), X66017 (SEQ ID No 18), JN594507 (SEQ ID No 19), DQ984181 (SEQ ID No 20), DQ318192 (SEQ ID No 21), AF302496 (SEQ ID No 22), AF302497 (SEQ ID No 23), AF302498 (SEQ ID No 24), L07843 (SEQ ID No 25), AF024635 (SEQ ID No 26), AF024634 (SEQ ID No 27), FJ719368 (SEQ ID No 28), FJ719369 (SEQ ID No 29) and nucleic acid sequence variants with at least 70% sequence identity to SEQ ID No 13 to 29
• or wherein the gene comprises a sequence which codes for an amino acid sequence according to SEQ ID No 66 to 82, or for an amino acid sequence variant with at least 85% sequence identity to SEQ ID No 66 to 82;
• and/or
• iii. at least one copy of a gene for encoding a cytochrome P450 monooxygenase, wherein the gene comprises a sequence selected from the group comprising nucleic acids according to accession number AB619802 (SEQ ID No 30), AB619803 (SEQ ID No 31), DQ335781 (SEQ ID No 32), JN565975 (SEQ ID No 33), XM_002331391 (SEQ ID No 34), XM_003525274 (SEQ ID No 35), JF803813 (SEQ ID No 36), XM_004139039 (SEQ ID No 37), GU997666 (SEQ ID No 38), JX036032 (SEQ ID No 39), XM_002522891 (SEQ ID No 40), AM457725 (SEQ ID No 41), XM_002265988 (SEQ ID No 42), XM_002527956 (SEQ ID No 43), BT147421 (SEQ ID No 44), XM_003530477 (SEQ ID No 45), BT096613 (SEQ ID No 46), XM_002309021 (SEQ ID No 47), BT051785 (SEQ ID No 48), XM_002513137 (SEQ ID No 49), XM_002264607 (SEQ ID No 50), XM_002324633 (SEQ ID No 51), XM_003531801 (SEQ ID No 52), XM_002280933 (SEQ ID No 53) and nucleic acid sequence variants with at least 70% sequence identity to SEQ ID No 30 to 53,
• or wherein the gene comprises a sequence which codes for an amino acid sequence according to SEQ ID No 83 to 105, or for an amino acid sequence variant with at least 85% sequence identity to SEQ ID No 83 to 105.