Production of xylitol from glucose by a recombinant strain
AssigneeRoquette Freres
InventorsASTRID SCHAEFER, MELANIE DIEFENBACHER, YIMING CHANG, SUMIRE HONDA MALCA, MARKUS SCHWAB, SOPHIE DEFRETIN, TANIA GERARD, ARNAUD HEYSEN, FRIEDERIKE THOR
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Abstract
The present invention relates to a recombinant microbial host for the production of xylitol, the recombinant microbial host containing a nucleic acid sequence encoding an NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product, and a nucleic acid sequence encoding an NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product.
Claims17
1-17. (canceled)
A recombinant host cell capable of producing xylitol, wherein said host cell comprises:
- an heterologous nucleic acid sequence encoding a NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product;
- and,
- an heterologous nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product.
Dependencies are inferred from the claim text.