Plant glutamine phenylpyruvate transaminase gene and transgenic plants carrying same
AssigneesLos Alamos National Security, LLC, University of Maine System Board of Trustees
InventorsPat J. Unkefer, Penelope S. Anderson, Thomas J. Knight
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Abstract
The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating growth-enhanced transgenic plants. In one embodiment, transgenic plants engineered to over-express glutamine phenylpyruvate transaminase (GPT) are provided.
Claims34
A method for producing a plant having enhanced growth properties relative to an analogous wild type or untransformed plant, comprising:
- (a) introducing into the plant a glutamine phenylpyruvate transaminase (GPT) transgene that encodes a GPT polypeptide having an amino acid sequence that has at least 80% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:
- 11, SEQ ID NO:
- 13, or SEQ ID NO:
- 15 and GPT catalytic activity;
- and,
- (b) expressing said GPT transgene in said transgenic plant or progeny thereof, wherein GPT polypeptides are overexpressed in said transgenic plant or said progeny thereof relative to an analogous wild type or untransformed plant of the same species,
- wherein said transgenic plant has an increased biomass yield relative to an analogous wild type or untransformed plant of the same species.
A method for generating and selecting transgenic plants having increased production of 2-oxo-glutaramate, comprising:
- (a) introducing into a plurality of plant cells a glutamine phenylpyruvate transaminase (GPT) transgene that encodes a GPT polypeptide having an amino acid sequence that has at least 80% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:
- 11, SEQ ID NO:
- 13, or SEQ ID NO:
- 15 and has GPT catalytic activity,
- (b) generating a plurality of transgenic plants from the plurality of plant cells;
- and,
- (c) selecting a transgenic plant from said plurality of transgenic plants that produces more 2-oxo-glutaramate relative to an analogous wild type or untransformed plant.
Dependencies are inferred from the claim text.