Microbial production of renewable glycolate
AssigneeMassachusetts Institute of Technology
InventorsGREGORY STEPHANOPOULOS, Zheng-Jun Li, Brian Pereira
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Abstract
Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.
Claims29
A cell engineered to produce glycolate, wherein the cell:
- has reduced or eliminated activity of, or reduced or eliminated expression of xylulokinase relative to a wild type cell,
- recombinantly expresses an enzyme that interconverts xylulose and ribulose,
- recombinantly expresses a D-ribulose-phosphate aldolase,
- recombinantly expresses a D-ribulokinase, and
- recombinantly expresses a glycolaldehyde dehydrogenase.
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An Escherichia coli cell engineered to produce glycolate, wherein the cell comprises a deletion of a xylB gene, recombinantly expresses a dte gene from Pseudomonas cichorii that is codon-optimized for E. coli, recombinantly expresses a fucA gene from E. coli, recombinantly expresses a fucK gene from E. coli, and recombinantly expresses an aldA gene from E. coli.
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Dependencies are inferred from the claim text.