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US 2017/0119873 A9Published application
Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
AssigneeThe Trustees of the University of Pennsylvania
InventorsJames M. Wilson, Guangping Gao, Soumitra Roy
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Abstract
A recombinant vector comprises simian adenovirus sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
Claims20
A recombinant adenovirus having a capsid comprising a SV39 hexon protein having the amino acid sequence of SEQ ID NO:
- 36, having packaged therein a nucleic acid sequence comprising (a) an adenovirus 5′ inverted terminal repeat sequence, (b) a nucleotide sequence heterologous to SV39 encoding a therapeutic or immunogenic product operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell, and (c) an adenovirus 3′ inverted terminal repeat sequence.
A synthetic simian adenovirus serotype nucleic acid sequence selected from one or more of the group consisting of:
- (a) 5′ inverted terminal repeat (ITR) sequences;
- (b) the adenovirus E1a region, or a fragment thereof selected from among the 13S, 12S and 9S regions;
- (c) the adenovirus E1b region, or a fragment thereof selected from among the group consisting of the small T, large T, IX, and IVa2 regions;
- (d) the E2b region;
- (e) the L1 region, or a fragment thereof selected from among the group consisting of the 28.1 kD protein, polymerase, agnoprotein, 52/55 kD protein, and IIIa protein;
- (f) the L2 region, or a fragment thereof selected from the group consisting of the penton, VII, VI, and Mu proteins;
- (g) the L3 region, or a fragment thereof selected from the group consisting of the VI, hexon, or endoprotease;
- (h) the 2a protein;
- (i) the L4 region, or a fragment thereof selected from the group consisting of the 100 kD protein, the 33 kD homolog, and VIII;
- (j) the E3 region, or a fragment thereof selected from the group consisting of E3 ORF1, E3 ORF2, E3 ORF3, E3 ORF4, E3 ORF5, E3 ORF6, E3 ORF7, E3 ORF8, and E3 ORF9;
- (k) the L5 region, or a fragment thereof selected from a fiber protein;
- (l) the E4 region, or a fragment thereof selected from the group consisting of E4 ORF7, E4 ORF6, E4 ORF4, E4 ORF3, E4 ORF2, and E4 ORF1;
- and
- (m) the 3′ ITR,
- of any of Pan5 SEQ ID NO:1;
- SV1 SEQ ID NO:
- 24;
- SV25 SEQ ID NO:
- 29;
- and SV39 SEQ ID NO:
- 34, or sequence complementary to any of (a) to (m).
18 more elements
Dependencies are inferred from the claim text.