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US 2017/0101665 A1Published application
Nucleic Acid Amplification With Integrated Multiplex Detection
AssigneeBioArray Solutions, Ltd.
InventorsSukanta BANERJEE, Michael SEUL, Hui HUANG
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Abstract
Compositions and methods of detecting multiple proteins of interest in a sample using arrays are provided herein.
Claims19
An array for detecting multiple proteins of interest in a sample, the array comprising:
- a. Protein capture agents bound to beads and;
- b. Encoded oligonucleotides bound to beads, wherein the proteins of interest are captured using the protein capture agents and wherein capture is detected using encoded antibodies, said antibodies encoded with a conjugated DNA tag sequence which is designed to be amplified and wherein the amplified tag sequence can be decoded upon interaction with the bound encoded oligonucleotides.
A method of comprising:
- a. Providing an array comprising:
- i. protein capture agents bound to a first set of beads;
- ii. encoded oligonucleotides bound to a second set of beads;
- b. Incubating , a sample containing a protein of interest with the array to allow the protein to bind the protein capture agent;
- c. Removing any unbound proteins from protein capture reagent;
- d. Incubating bound proteins with encoded antibodies to form a protein capture agent:protein:encoded antibody complex wherein said encoded antibody is conjugated to a DNA tag sequence capable of identifying the bound protein once decoded;
- e. Removing any unbound encoded proteins from the protein capture reagent:protein sample;
- f. Transcribing the conjugated DNA from the complex with RNA polymerase;
- g. Generating cDNA of the transcribed DNA using reverse transcriptase;
- h. Incubating the complex under conditions to allow hybridization of the cDNA to the encoded nucleotides bound to the second set of beads;
- i. Determining the presence of the protein of interest from said hybridization.
8 more elements
A device for housing an array comprising at least 2 sections further comprising:
- a. A first section housing a microparticle array;
- and
- b. A second section housing a sample area for red blood cells, wherein said first section and said section are joined by a channel.
Dependencies are inferred from the claim text.