Prion free nanoparticle compositions and methods of making thereof
AssigneeAbraxis BioScience, LLC
InventorsNeil P. DESAI, Viktor PEYKOV, Patrick SOON-SHIONG
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Abstract
The present invention provides prion-free compositions comprising nanoparticles comprising albumin and substantially water insoluble drugs. Also provided are methods of making prion-free compositions and methods of removing prion proteins from the nanoparticle compositions. Methods of using the compositions, as well as kits useful for carrying out the methods are also provided.
Claims22
A composition comprising nanoparticles comprising albumin and a substantially water insoluble pharmacologically active agent, wherein the composition is substantially free of a prion protein.
A composition comprising nanoparticles comprising albumin and a substantially water insoluble pharmacologically active agent, wherein the albumin in the composition was obtained by a method comprising a prion removal process, said prion removal process comprising contacting an initial albumin composition with a ligand capable of binding to a prion protein.
A method of producing a composition comprising nanoparticles comprising albumin and a substantially water insoluble pharmacologically active agent, said method comprising:
- a) removing a prion protein from an initial albumin composition;
- b) subjecting a mixture comprising a solution comprising the prion-removed albumin and an organic phase comprising said substantially water insoluble pharmacologically active agent dispersed in an organic solvent to a high shear condition.
A method of producing a composition comprising nanoparticles comprising albumin and a substantially water insoluble pharmacologically active agent, comprising:
- a) subjecting a mixture comprising an organic phase comprising said substantially water insoluble pharmacologically active agent and an albumin solution to a high shear condition, and b) removing a prion protein from said mixture.
A method of removing a prion protein from a composition suspected of containing a prion protein comprising nanoparticles comprising albumin and a substantially water insoluble pharmacologically active agent, comprising:
- a) contacting the composition with a ligand capable of binding to a prion protein, b) removing the ligand and proteins bound thereto from the composition.
Dependencies are inferred from the claim text.