Methods and reagents for analyzing protein-protein interfaces
AssigneeWarp Drive Bio, Inc.
InventorsGregory L. VERDINE, M. James NICHOLS, Sharon A. TOWNSON, Uddhav Kumar SHIGDEL, Seung-Joo LEE, Dylan T. STILES, Neville J. ANTHONY
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Abstract
The present disclosure provides methods and reagents useful for analyzing protein-protein interfaces such as interfaces between a presenter protein (e.g., a member of the FKBP family, a member of the cyclophilin family, or PIN1) and a target protein. In some embodiments, the target and/or presenter proteins are intracellular proteins. In some embodiments, the target and/or presenter proteins are mammalian proteins.
Claims20
A compound comprising:
- (a) an FKBP binding moiety or cyclophilin binding moiety;
- and
- (b) a cross-linking group, wherein the cross-linking group is a sulfhydryl-reactive cross-linking group, an amino-reactive cross-linking group, a carboxyl-reactive cross-linking group, a carbonyl-reactive cross-linking group, or a triazole-forming cross-linking group.
A method of identifying a target protein which binds to a presenter protein, the method comprising:
- (a) providing (i) a compound comprising a presenter protein binding moiety and a cross-linking moiety;
- (ii) a target protein;
- and (iii) a presenter protein;
- (b) combining the compound, the target protein, and the presenter protein under conditions suitable to permit complex formation if the compound is capable of forming a complex with the presenter protein;
- and
- (c) determining whether the target protein binds to the presenter protein in the complex, wherein if the target protein binds to the presenter protein, the target protein is identified as a target protein that binds to a presenter protein.
Dependencies are inferred from the claim text.