Bioactive compositions and methods for their preparation and use
AssigneeBioregenerative Sciences
InventorGreg Maguire
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Abstract
A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.
Claims25
1-16. (canceled)
A composition comprising extracellular products secreted by cells of (1) an adult human adipose derived stem cell (ADSC) line, (2) a fetal human fibroblast cell line (HDF-f), and (3) a hair dermal papilla (HDP) cell line.
A method for treating a hair condition in a subject in need thereof comprising administering to said subject a therapeutically effective amount of a composition prepared by
- culturing cells of a first cell line, a second cell line, and a third cell line in one or more culture media, wherein said cultured cells secrete extracellular products into said culture media so that one or more conditioned culture media are formed;
- separating said one or more conditioned culture media from respective said cultured cells;
- and
- combining said one or more conditioned culture media to form the composition,
- wherein said first, second, and third cell lines are an adult human adipose derived stem cell (ADSC) line, a fetal human fibroblast cell line, and a hair dermal papilla (HDP) cell line, respectively.
26-30. (canceled)
Dependencies are inferred from the claim text.