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US 2017/0088863 A1Published application
Recombinant Host Cells Comprising Phosphoketalase
AssigneeButamax Advanced Biofuels LLC
InventorsMichael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Abstract
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Claims21
1-35. (canceled)
A recombinant host cell comprising:
- (i) at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity;
- (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity;
- and
- (iii) at least one genetic modification in an endogenous gene encoding a polypeptide having glycerol-3-phosphate activity.
1 more element
Dependencies are inferred from the claim text.