Methods Of Propagating Rhinovirus C In Previously Unsusceptible Cell Lines
AssigneeWisconsin Alumni Research Foundation
InventorsJames E. Gern, Yury A. Bochkov, Ann C. Palmenberg
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Abstract
The present invention provides methods of propagating rhinovirus C (RV-C) in a host cell; a host cell comprising an effective amount of a heterologous CDHR3 receptor such that the host cell can support propagation of rhinovirus C; and kits comprising at least one host cell previously unable to support rhinovirus C growth, wherein the host cell comprises a heterologous CDHR3 receptor and a sample of rhinovirus C. Methods of use are also provided.
Claims29
A method of propagating rhinovirus C or rhinovirus C variant, the method comprising the steps of:
- a. obtaining a host cell comprising at least one heterologous CDHR3 receptor;
- and
- b. infecting the host cell with a sample of rhinovirus C or rhinovirus C variant;
- wherein the rhinovirus C propagates.
A kit comprising:
- a. at least one host cell able to support rhinovirus C replication, wherein the host cell comprises a heterologous CDHR3 receptor;
- and
- b. a sample of rhinovirus C or rhinovirus C variant.
A kit comprising:
- a. a nucleic acid encoding a CDHR3 receptor;
- and
- b. a sample of rhinovirus C.
A host cell infected with rhinovirus C or rhinovirus C variant, wherein the host cell has been modified to express an effective amount of a CDHR3 receptor and support propagation of rhinovirus C.
A method of screening for antiviral drugs, the method comprising the steps of
- a. Exposing a test compound to a host cell comprising at least one heterologous CDHR3 receptor and a sample of rhinovirus C or a rhinovirus C variant;
- and
- b. Determining whether the test compound interferes with propagation of the rhinovirus.
A method of screening for antiviral drugs, the method comprising the steps of:
- a. Exposing a test compound to a sample of rhinovirus C or a rhinovirus C variant;
- and
- b. Determining whether the test compound interferes with propagation of the rhinovirus in a host cell comprising at least one heterologous CDHR3 receptor.
A method of screening for inhibitor treatments, the method comprising the steps of:
- a. Exposing a candidate inhibitor treatment to a host cell comprising at least one heterologous CDHR3 receptor and a sample of rhinovirus C or a rhinovirus C variant;
- and
- b. Determining whether the treatment interferes with propagation of the rhinovirus.
A method of screening for inhibitor treatments, the method comprising the steps of:
- a. Exposing a candidate treatment to a sample of rhinovirus C or a rhinovirus C variant;
- and
- b. Determining whether the treatment interferes with propagation of the rhinovirus in a host cell comprising at least one heterologous CDHR3 receptor.
Dependencies are inferred from the claim text.