Engineered biocatalysts useful for carbapenem synthesis
AssigneeCodexis, Inc.
InventorsJoly Sukumaran, Derek Smith, Hong Yang, Wan Lin Yeo, Jeffrey C. Moore
Life of this patent
Estimating the term…
Abstract
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.
Claims20
A engineered polynucleotide encoding an engineered pNB esterase polypeptide having pNB esterase activity, wherein said pNB esterase polypeptide comprises an amino acid sequence having at least 90% identity to SEQ ID NO:
- 2 and one or more residue differences as compared to SEQ ID NO:2 at residue positions selected from:
- 108, 115, 116, 130, 193, 214, 219, 273, 276, 321, and 362, wherein the differences at positions 115 and 273 are 115Q/W and 273A/E/TN, respectively, and optionally wherein said engineered polypeptide further comprises a different residue at position 362.
A engineered polynucleotide comprising a nucleotide sequence having at least 90% sequence identity to a polynucleotide sequence selected from SEQ ID NO:
- 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, and 119.
Dependencies are inferred from the claim text.