US 2017/0067066 A1 · CELL WITH REDUCED PPGPPASE ACTIVITY

Abstract

The present invention relates to a cell which is genetically modified over its wild type in such a way that it has a reduced ppGppase activity relative to its wild type, and preferably an essential amino acid, even more preferably an essential amino acid derived from serine, most preferably methionine or tryptophan, to a feed additive comprising such a cell, to a method of preparing a cell overproducing essential amino acid, more preferably an essential amino acid derived from serine, most preferably methionine or tryptophan, comprising preparing a cell having a knocked-out gene coding for a ppGppase, and to a method of preparing an essential amino acid, more preferably an essential amino acid derived from serine, most preferably methionine or tryptophan, comprising the steps of a) culturing the cell according to the first aspect of the present invention or to any of its embodiments, and b) optionally: purifying the amino acid.

Claims16

Dependent claims

1-19. (canceled)

An Escherichia coli cell, wherein said Escherichia coli cell overproduces an essential amino acid derived from serine, wherein the activity of at least one ppGppase selected from the group consisting of the amino acid sequences SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6 is reduced as a result of said ppGppase having the following modification:

an insertion of the two amino acid His and Asn is present between Asp84 and Met85,
and wherein the Escherichia coli cell has a (p)ppGppase-synthetase activity which is essentially unchanged relative to its wild type.

Dependencies are inferred from the claim text.

Facts

Application

15/357,482Filed 21 Nov 2016

Priority

EP 12156052.817 Feb 2012

Published

9 Mar 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
A23K 10/12
Obtained by microbiological or biochemical processes: by fermentation of natural products, e.g. of vegetable material, animal waste material or…
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 9/16
Hydrolases: acting on ester bonds

Cell with reduced ppgppase activity

Life of this patent

Cell with reduced ppgppase activity

Life of this patent

Abstract

Claims16