Chimeric protein toxins for expression by therapeutic bacteria
OwnerDavid Gordon Bermudes, David Quinteroinventors
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Abstract
Bacteria with tumor-targeting capability express, surface displayed, secreted and/or released modified chimeric therapeutic proteins with enhanced therapeutic activity against a neoplastic tissue including solid tumors, lymphomas and leukemias. The bacteria may be attenuated, non-pathogenic, low pathogenic or a probiotic. The chimeric proteins may be protease sensitive and may optionally be further accompanied by co-expression of a secreted protease inhibitor as a separate molecule or as a fusion.
Claims20
A genetic construct which causes a live host bacterium to express a peptide selected from the group consisting of at least one of:
- (a) a reorganized polycistronic cytolethal distending toxin fusion with antineoplastic activity expressed or secreted in an absence of sty and ttsa, (b) a modified chimeric cytolethal distending toxin:apoptin fusion with antineoplastic activity expressed or secreted in an absence of in an absence of sty and ttsa, and (c) a functionally active modified chimeric Pseudomonas ToxA therapeutic molecule with antineoplastic activity.
A chimeric protease inhibitor comprising YebF fused to sunflower trypsin inhibitor, adapted to inhibit at least one serine protease.
A method for treating neoplastic tumor cells within a living organism, comprising:
- administering to a human or animal a live genetically engineered bacterium expressing peptide selected from the group consisting of at least one of:
- (a) a reorganized polycistronic cytolethal distending toxin fusion with antineoplastic activity expressed or secreted in an absence of sty and ttsa, (b) a modified chimeric cytolethal distending toxin:apoptin fusion with antineoplastic activity expressed or secreted in an absence of in an absence of sty and ttsa, and (c) a functionally active modified chimeric Pseudomonas ToxA therapeutic molecule with antineoplastic activity, wherein said peptide is secreted or released by said bacterium in a functionally active form.
Dependencies are inferred from the claim text.