Selection in Fungi
AssigneeNovozymes A/S
InventorCarsten Lillelund Olsen
Life of this patent
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Abstract
The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
Claims48
1-12. (canceled)
A polynucleotide construct suitable for transformation into a fungal host cell and integration into the genome of said cell, said construct comprising:
- (a) a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 1 to 5 nucleotides between its branch site and its acceptor site;
- and
- (b) a second polynucleotide encoding a polypeptide of interest.
Dependencies are inferred from the claim text.