Tagged ligands for enrichment of rare analytes from a mixed sample
AssigneeGPB Scientific, LLC
InventorsAllyn Forsyth, Helen Barnes
Life of this patent
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Abstract
Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.
Claims16
-12. (canceled)
A method for enriching rare analytes from a sample comprising:
- a. contacting said sample with an affinity-tagged ligand (ATL) wherein said ATL comprises:
- i. an affinity tag comprising a magnetic particle, and
- ii. a first ligand and a second ligand different from said first ligand, wherein said first ligand and said second ligand are linked to said affinity tag, wherein each of said first ligand and said second ligand selectively binds one or more markers of said rare analytes, thereby labeling said rare analytes with said affinity-tagged ligand;
- b. flowing said sample comprising said labeled rare analytes through a microfluidic device comprising an array of obstacles configured to laterally displace components in said sample;
- and
- c. flowing said sample comprising said labeled rare analytes through an enrichment device comprising a surface functionalized with a capture moiety, wherein said capture moiety comprises a magnet or magnetized substrate, thereby selectively enriching said rare analytes.
Dependencies are inferred from the claim text.