Microorganisms for the production of aniline
AssigneeGenomatica, Inc.
InventorPriti Pharkya
Life of this patent
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Abstract
A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.
Claims17
1-46. (canceled)
A non-naturally occurring Escherichia coli, comprising an aniline pathway, said aniline pathway comprising an anthranilate synthase, a 3-dehydroquinate synthase, a 3-dehydroquinate dehydratase, a shikimate dehydrogenase or a quinate/shikimate dehydrogenase, a shikimate kinase, a 3-phosphoshikimate-1-carboxyvinyltransferase, a chorismate synthase and an anthranilate decarboxylase, wherein said non-naturally occurring Escherichia coli, comprises at least two exogenous nucleic acids, the two exogenous nucleic acids encoding enzymes selected from the group consisting of the 3-dehydroquinate synthase, the anthranilate synthase, the 3-dehydroquinate dehydratase, the shikimate dehydrogenase or the quinate/shikimate dehydrogenase, the shikimate kinase, the 3-phosphoshikimate-1-carboxyvinyltransferase, and the chorismate synthase, wherein an endogenous nucleic acid encodes the anthranilate decarboxylase, and wherein the aniline pathway enzymes are expressed in a sufficient amount to produce aniline.
Dependencies are inferred from the claim text.