Mutant Microorganism Comprising Gene Encoding Methylmalonyl-CoA Reductase and Use Thereof
AssigneeSK Innovation Co., Ltd.
InventorsYoung Bin Seo, Ki Sung Lee, Jae Yeon Park, Joong Min Park, Woo Chan Park, Jong Joo Lee, Hye Sung Lee, Kyeong Hyeon Kang
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Abstract
Provided herein is a mutant microorganism containing a methylmalonyl-CoA reductase-encoding gene having an activity of converting methylmalonyl-CoA to methylmalonate semialdehyde and uses of the mutant microorganism. The mutant microorganism includes a gene encoding kingdom Archaea-derived methylmalonyl-CoA reductase.
Claims8
A mutant microorganism derived from a microorganism having the ability to produce succinyl-CoA from a carbon source, wherein the mutant microorganism contains genes encoding the following enzymes and has the ability to produce 3-HIBA (3-hydroxyisobutyric acid):
- (i) methylmalonyl-CoA mutase;
- (ii) methylmalonyl-CoA epimerase;
- (iii) methylmalonyl-CoA reductase;
- and
- (iv) 3-hydroxyisobutyrate dehydrogenase, wherein the enzyme of (iii) is an enzyme exhibiting methylmalonyl-CoA reductase activity among enzymes having malonyl-CoA reductase activity.
Dependencies are inferred from the claim text.