Cytomegalovirus vaccines and methods of production
AssigneeThe Trustees of Princeton University
InventorsThomas Shenk, Dai Wang
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Abstract
Methods of increasing diversity in cytomegalovirus vaccines through the selection of cell type in which the virus is propagated, and the use of cytomegalovirus produced by those methods in the development of vaccine compositions, are disclosed. Vaccine compositions comprising CMV isolated from epithelial cells are also disclosed.
Claims14
A method of making an immunogenic composition comprising a population of live attenuated, inactivated or killed cytomegaloviruses (CMVs), or virion components thereof, comprising:
- a) passaging a single strain or isolate of CMV having a gene encoding a functional pUL131 protein in a fibroblast cell culture to thereby produce a passaged strain or isolate of CMV;
- b) amplifying the passaged strain or isolate of CMV in an epithelial cell culture to thereby produce an amplified strain or isolate of CMV;
- c) harvesting the amplified strain or isolate of CMV from the epithelial cell culture to thereby obtain cell-type conditioned CMV;
- and
- d) combining the cell-type conditioned CMV with a pharmaceutically acceptable carrier or adjuvant,
- thereby making an immunogenic composition comprising a population live attenuated, inactivated or killed CMVs, or virion components thereof.
A method of inducing an immune response against CMV in an individual in need thereof, comprising administering to the individual an immunogenic composition comprising a cytomegalovirus (CMV) population, or virion components thereof, admixed with a pharmaceutically acceptable carrier or adjuvant, wherein the CMV population includes CMV that have been passaged in a fibroblast and amplified in an epithelial cell.
A kit comprising a container in which is contained:
- a) one or CMV strains or clinical isolates;
- b) cultured cells of one or more selected cell types;
- and
- c) instructions for using the cultured cells and the CMV strains or isolates to produce cell type-conditioned CMV for use in a CMV vaccine.
Dependencies are inferred from the claim text.